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Improved processes for the production and isolation of dynemicin A and large-scale fermentation in a 10000-liter fermentor |
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| Authors: | Kin Sing Lam Jeffrey A Titus Thomas T Dabrah Dennis L Kimball Jacqueline M Veitch Donald R Gustavson Bruce J Compton James A Matson Salvatore Forenza John Ross David Miller John Roach John Beutler |
| Institution: | (1) Department 114, Bristol-Myers Squibb Company, Pharmaceutical Research Institute, P.O. Box 5100, 06492 Wallingford, CT, USA;(2) Industrial Division, Bristol-Myers Squibb Company, Syracuse, NY, USA;(3) Frederick Cancer Research and Development Center, Frederick, MD, USA |
| Abstract: | Summary Supplementing the culture ofMicromonospora chersina sp. nov. No. M956-1 with NaI (0.5 mg/l) enhanced the production of dynemicin A by 35-fold in shake flask culture. Homogeneous dynemicin A was obtained from the whole broth extract by Dicalite chromatography, Sephadex LH-20 chromatography and vacuum liquid chromatography. Gram quantities of dynemicin A were obtained from the fermentation ofM. chersina sp. nov. No. M956-1 in a 10000-liter fermentor. |
| Keywords: | Antitumor antibiotic Dynemicin A Micromonospora chersina |
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