NMR investigations of protein-carbohydrate interactions: refined three- dimensional structure of the complex between hevein and methyl beta- chitobioside |
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| Authors: | Asensio, JL Canada, FJ Bruix, M Gonzalez, C Khiar, N Rodriguez-Romero, A Jimenez-Barbero, J |
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| Affiliation: | Instituto de Quimica Organica General, CSIC, Madrid, Spain. |
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| Abstract: | The specific interaction of hevein with GlcNAc-containing oligosaccharideshas been analyzed by1H-NMR spectroscopy. The association constants for thebinding of hevein to a variety of ligands have been estimated from1H-NMRtitration experiments. The association constants increase in the orderGlcNAc-alpha(1-->6)-Man < GlcNAc < benzyl-beta-GlcNAc |
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