Relationships within the Melanogaster subgroup species of the genus Drosophila (Sophophora)

Five members of the melanogaster species subgroup of the subgenus Sophophora have been studied cytologically, their mitotic chromosomes analysed after Giemsa, C-banding and quinacrine staining. In all five species (D. yakuba, D. teissieri, D. erecta, D. orena and D. mauritiana) n=4 and all of the species except D. orena have a typical melanogaster like mitotic karyotype though there are clear differences between species in the distribution of both C⁺ and Q⁺ material. D. orena has large metacentric X and Y chromosomes due to the accumulation of intensively fluorescing material on these elements with respect to their homologues in melanogaster. This extra heterochromatin of D. orena correlates with a very high proportion of satellite DNA in its nuclear genome (S. Barnes, unpublished). The polytene chromosomes of these species were studied after quinacrine staining and Q⁺ material found to be restricted to the polytene fourth chromosomes, with the exception of D. orena which possesses considerable Q⁺ material in its chromocentre. These findings are discussed in the light of other studies of karyotype evolution in the genus Drosophila.