A point mutation in the chloroplast rps12 gene from Nicotiana plumbaginifolia confers streptomycin resistance |
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Authors: | Chen-Mei Hsu Wen-Pei Yang Chi-Chang Chen Yiu-Kai Lai Tsai-Yun Lin |
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Affiliation: | (1) Institute of Life Science, National Tsing Hua University, 30043 Hsinchu, Taiwan, ROC;(2) Department of Botany, National Taiwan University, 10764 Taipei, Taiwan, ROC |
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Abstract: | In an effort to understand the mechanism of streptomycin resistance in Nicotiana plumbaginifolia, we have sequenced the chloroplast rps12 gene, a potential molecular target. We report that a streptomycin-resistant mutant isolated from protoplast cultures of N. plumbaginifolia contains an A-to-G transition at nucleotide position 149 in exon 2 of the chloroplast rps12 gene. The detected point mutation predicts a substitution of arginine for lysine in a phylogenetically conserved region. |
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Keywords: | Nicotiana plumbaginifolia streptomycin resistance chloroplast DNA rps12 |
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