Cytotoxic abietane diterpenes from Hyptis martiusii Benth |
| |
Authors: | da Araújo Edigênia Cavalcante Cruz Lima Mary Anne S Montenegro Raquel C Nogueira Marcelle Costa-Lotufo Letícia V Pessoa Cláudia de Moraes Manoel Odorico Silveira Edilberto R |
| |
Institution: | Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Brazil. |
| |
Abstract: | From roots of Hyptis martiusii Benth. two tanshinone diterpenes were isolated, the new 7beta-hydroxy-11,14-dioxoabieta-8,12-diene (1) in addition to the known 7alpha-acetoxy-12-hydroxy-11,14-dioxoabieta-8,12-diene (7alpha-acetoxyroyleanone) (2). Structures of 1 and 2 were established by spectroscopic means. The cytotoxic activity against five cancer cell lines was evaluated. Compounds 1 and 2 displayed considerable cytotoxic activity against several cancer cell lines with IC50 values in the range of 3.1 to 11.5 microg/ml and 0.9 to 7.6 microg/ml, respectively. The cytotoxic activity seemed to be related to inhibition of DNA synthesis, as revealed by the reduction of 5-bromo-2'-deoxyuridine incorporation and induction of apoptosis, as indicated by the acridine orange/ethidium bromide assay and morphological changes after 24 h of incubation in leukemic cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|