Rapid Staining of Nuclei and Chromosomes in Root Tips |
| |
Authors: | Morton W Miller Gladys Yettewich |
| |
Institution: |
a Department of Radiation Biology and Biophysics, School of Medicine and Dentistry, The University of Rochester, Rochester, New York |
| |
Abstract: | Feulgen reagent quickly heated to and maintained at 60 C just before immersion of plant material, basic fuchsin in acid alcohol at room temperature, and pinacyanol at room temperature will stain hydrolyzed root tip nuclei and chromosomes in one minute or less. This technic, coupled with fast fixation, can be utilized when uncertainties exist as to when to begin sampling plant meristem cells for mitoses or when time does not allow for standard fixation and Schiff staining. |
| |
Keywords: | |
本文献已被 InformaWorld 等数据库收录! |
|