Protective role of <Emphasis Type="SmallCaps">l</Emphasis>-ascorbic acid on antioxidant defense system in erythrocytes of albino rats exposed to nickel sulfate |
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Authors: | Kusal K Das Amrita Das Gupta Salim A Dhundasi Ashok M Patil Swastika N Das Jeevan G Ambekar |
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Institution: | (1) Department of Physiology, Al-Ameen Medical College, Bijapur, 586108, Karnataka, India;(2) Department of Pathology, Al-Ameen Medical College, Bijapur, 586108, Karnataka, India;(3) Department of Engineering Chemistry, B.L.D.E.A’s. Dr. P.G. Halakatti College of Engineering and Technology, Bijapur, 586103, Karnataka, India;(4) Department of Biochemistry, Sri B.M. Patil Medical College, Bijapur, 586103, Karnataka, India |
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Abstract: | In this experimental study, we investigated whether l-ascorbic acid has any influence on the blood antioxidant defense system, lipid peroxidation and hematological parameters
of the albino rats exposed to nickel sulfate(NiSO4).Twenty four adult rats were divided into four groups of six animals in each group. The control rats were untreated and comprised
Group I. Group II rats were administered nickel sulfate (2.0 mg/100 g b.wt.; intraperitonially, i.p.). Group II rats were
treated orally l-ascorbic acid (50 mg/100 g b.wt.) and Group IV rats were given both nickel sulfate and l-ascorbic acid simultaneously on alternate days until the tenth dose. The hematological parameters were assessed: red blood
corpuscle counts, packed cell volume %, hemoglobin concentration, white blood corpuscle counts and platelets count decreased
significantly and clotting time increased significantly in nickel treated rats. We also observed increase malondialdehyde
(MDA) and decrease glutathione level (GSH) in erythrocytes of nickel treated rats. The activities of erythrocyte antioxidant
enzymes like superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were significantly increased in
rats treated with nickel sulfate. Simultaneously treatment of l-ascorbic acid exhibited a possible protective role on the toxic effect of nickel sulfate on the hematological values, erythrocyte
MDA and GSH concentrations as well as antioxidant enzymatic defense system. |
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Keywords: | nickel sulfate l-ascorbic acid" target="_blank">l-ascorbic acid hematology erythrocyte antioxidant system |
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