Effects of degree of cell-cell contact on liver specific functions of rat primary hepatocytes |
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Authors: | Sung Mun Yang Doo Hoon Lee Jung Keug Park |
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Institution: | (1) Department of Chemical Engineering, Dongguk University, 100-715 Seoul, Korea |
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Abstract: | Cell-cell interaction and the extracellular matrix (ECM) are believed to play essential roles duringin vitro culturing of primary hepatocytes in the control of differentiation and in the maintenance of tissue specific functions. The
objective of this study was to examine the effects of degree of cell-cell contact (DCC) on liver specific function of rat
primary hepatocytes. Hepatocyte aggregates with various degrees of cell-cell contact,i.e., dispersed cells, longish aggregate, rugged aggregate, and smooth spheroid were obtained at 1, 5–6, 15–20, and 36–48 hrs,
respectively in suspension cultures grown in spinner flasks embedded in Caalginate bead and collagen gel in order. The smooth
spheroids displayed a decrease in viability and functional activities. This may result from mass transfer limitation and shear
damage caused by agitation during aggregation. The rugged aggregate showed a higher viability and albumin secretion rate than
the dispersed cells or the other aggregates. This result indicates the possible enhancement of a bioartificial liver's (BAL)
performance using primary hepatocytes and the reduction in time to prepare a BAL through optimization of the immobilization
time. |
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Keywords: | collagen alginate cell-cell interaction hepatocyte aggregate hepatocyte spheroid |
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