Some aspects of fluorescence and microcalorimetric studies of cytochrome C oxidase |
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Authors: | A Musatov E A Permyakov J Bagelova L Morozova V I Snyrov |
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Institution: | Laboratory of Biophysics, Institute of Experimental Physics, Slovac Acad. Sci., Kosice. |
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Abstract: | Effects of the solubilizing detergent type, pH and temperature on the structure of cytochrome c oxidase have been studied by the intrinsic fluorescence and scanning microcalorimetry methods. The data obtained allow to conclude that the enzyme solubilization by lauryl maltoside gives a more native preparation in comparison with that obtained by solubilization in Tween 80. |
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