Enhanced production of scopolin bySolanum aviculare cells immobilised within Ca-alginate gel beads |
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| Authors: | C Roisin F Gillet-Manceau J E Nava Saucedo M Fliniaux A Jacquin-Dubreuil J -N Barbotin |
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| Institution: | (1) Laboratoire de Génie Cellulaire (EA 906), Université de Picardie Jules Verne, Faculté des Sciences, 33, rue St.-Leu, F-80039 Amiens Cedex, France;(2) Laboratoire de Pharmacognosie et Phytotechnologie (EA 908), Université de Picardie Jules Verne, Faculté de Pharmacie, 1, rue des Louvels, F-80037 Amiens Cedex 1, France |
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| Abstract: | Summary Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour ofS. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics ofSolanum aviculare cells immobilised within Ca-alginate beads were notably different from those of suspended cells. ImmobilisedS. aviculare have accumulated scopolin (up to 120  g·g–1 FWB) within beads and excreted it into the culture medium (up to 8 g·g–1 FWB). Contrary to suspended cells which have accumulated only traces of this metabolite within intracellular compartments (1 g·g–1 FWB), no scopolin has been found into the culture medium.Abbreviations ANA
 -Naphthaleneacetic acid
- HPLC
high performance liquid chromatography
- FWB
fresh weight biomass
- LS medium
Linsmaier and Skoog medium
- MS
mass spectroscopy
- NMR
nuclear magnetic resonance
- r2
coefficient of determination
- s
standard deviation |
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