| 青海湖裸鲤mtDNA遗传多样性的初步研究 |
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| 引用本文： | 赵凯,李军祥,张亚平,罗静,李太平,吴华,田海宁ZHAO Kal,LI Jun-xiang,ZHANG Ya-ping,LUO Jing,LI Tai-ping,WU Hua,TIAN Hai-ning.青海湖裸鲤mtDNA遗传多样性的初步研究[J].遗传,2001,23(5):445-633. |
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| 作者姓名： | 赵凯 李军祥 张亚平 罗静 李太平 吴华 田海宁ZHAO Kal LI Jun-xiang ZHANG Ya-ping LUO Jing LI Tai-ping WU Hua TIAN Hai-ning |
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| 作者单位： | 1.青海大学农牧学院动物科学系,西宁 810003
2.中国科学院昆明动物所细胞与分子进化研究开放实验室,昆明 650223
1.College of Agriculture and Animal Husbandry,Qinghai University,Qinghai,Xining 810003,China;
2.Laboratory of Cellular and Molecular Euolution,Kunming Institute of Zoologythe Chinese Academy of Sciences,Yunnan,Kunming 650223,China |
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| 摘 要： | 本文用BclⅠ、AvaⅠ、BamHⅠ、PstⅠ、KpnⅠ、PvuⅡ共6种限制性内切核酸酶,分析了15尾青海湖裸鲤mtDNA的限制性片段长度多态性,共检测出20个酶切位点,发现BclⅠ、BamHⅠ和PvuⅡ三种酶切类型具有多态性.根据不同个体mtDNA的酶切类型,青海湖裸鲤存在4种mtDNA单倍型,计算mtDNA多态度π值为0.0043,初步认为青海湖裸鲤在线粒体DNA上存在较丰富的群体内变异、
Abstract:An analysis of patterns of cleavage of mtDNA by restriction endonucleases was performed for 15 Gymnocypris przewalskii przewalskii.Mitochondrial DNA polymorphisms were detected in the restriction patterns generated by the following 5 enzymes,BclI,AvaI,BamH],PstI,KpnI,PvuII.Only 3 of them (BclI,BantHI,PvuII) were found to be polymorphisms.Our results shorted that there were 4 mtDNA haplotypes in Gyrnnocypris przewalskii przewalskii and the genetic divergenec(π)was 0.0043,which indicated that mtDNA genetic diversity in Gymnocypris przewalskii przewalskii is higher.
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| 关 键 词： | 青海湖裸鲤 mtDNA 遗传多样性 Key words 限制性片段长度多态性 Qinghai lake Naked carps |
Mitochondrial DNA Diversity in Naked Carps in Qinghai-lake (Gymnocypris prezwalskii prezwalskii) |
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| ZHAO Kal,LI Jun-xiang,ZHANG Ya-ping,LUO Jing,LI Tai-ping,WU Hua,TIAN Hai-ning.Mitochondrial DNA Diversity in Naked Carps in Qinghai-lake (Gymnocypris prezwalskii prezwalskii)[J].Hereditas,2001,23(5):445-633. |
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| Authors: | ZHAO Kal LI Jun-xiang ZHANG Ya-ping LUO Jing LI Tai-ping WU Hua TIAN Hai-ning |
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