The Establishment of an In Vivo HIV-1 Infection Model in Humanized B-NSG Mice |
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Authors: | Fan Tian-Jiao Sun Li Yang Xian-Guang Jin Xia Sun Wei-Wei Wang Jian-Hua |
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Institution: | 1.CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China2.University of Chinese Academy of Sciences, Beijing 100039, China3.College of Life Science, Henan Normal University, Xinxiang 453007, China |
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Abstract: | Suitable animal models for human immunodeficiency virus type 1(HIV-1) infection are important for elucidating viral pathogenesis and evaluating antiviral strategies in vivo. The B-NSG(NOD-PrkdcscidIl2rgtm1/Bcge) mice that have severe immune defect phenotype are examined for the suitability of such a model in this study. Human peripheral blood mononuclear cells(PBMCs) were engrafted into B-NSG mice via mouse tail vein injection, and the repopulated human T-lymphocytes were observed at as early as 3-weeks post-transplantation in mouse peripheral blood and several tissues.The humanized mice could be infected by HIV-1, and the infection recapitulated features of T-lymphocyte dynamic observed in HIV-1 infected humans, meanwhile the administration of combination antiretroviral therapy(cART) suppressed viral replication and restored T lymphocyte abnormalities. The establishment of HIV-1 infected humanized B-NSG mice not only provides a model to study virus and T cell interplays, but also can be a useful tool to evaluate antiviral strategies. |
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Keywords: | Human immunodeficiency virus type 1 (HIV-1) Animal model Acute infection B-NSG mice |
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