Antibody-mediated targeting of liposomes to erythrocytes in the whole blood |
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| Affiliation: | 1. Division of Cardiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA;2. Division of Endocrinology, Diabetes, and Bone Disease, Icahn School of Medicine at Mount Sinai, New York, NY, USA;3. Basepaws Inc., Redondo Beach, CA, USA;4. Department of Nutritional Sciences and Diabetes Research Center, University of Alabama at Birmingham, Birmingham, AL, USA;6. Geriatric Research Education and Clinical Center, Birmingham Veterans Affairs Medical Center, Birmingham, AL, USA;1. Ios and Coleman Medicina Futura Medical Center, Naples, Italy;2. Diabetes Division, University of Texas Health Science Center, San Antonio, TX, USA;3. Institute of Clinical Physiology of the National Research Council (CNR), Pisa, Italy;4. NNF Center for Basic Metabolic Research and Department of Biomedical Sciences, The Panum Institute, University of Copenhagen, Copenhagen, Denmark;1. Lunenfeld-Tanenbaum Research Institute, Mt. Sinai Hospital, LTRI, 600 University Avenue TCP5-1004, Toronto, ON M5G 1X5, Canada;2. INSERM U1065, Mediterranean Center of Molecular Medicine, University Côte d''Azur, Faculty of Medicine, 06204 Nice, France;3. Department of Medicine, University of Toronto, Toronto, ON M5S 2J7, Canada;1. Department of Radiation Oncology, Seoul National University Bundang Hospital, Republic of Korea;2. Department of Surgery, Seoul National University, College of Medicine, Republic of Korea;3. Department of Radiation Oncology, Seoul National University, College of Medicine, Republic of Korea |
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| Abstract: | F(ab′)2 fragments derived from anti-rat erythrocyte antibody or normal rabbit serum IgG were covalently attached to the surface of liposomes consisting of equimolar amounts of egg phosphatidylcholine and cholesterol. These liposomes were interacted with rat, monkey or mouse blood, and their binding to both red and white blood cells was determined. Results of these studies show that coupling of liposomes to anti-rat erythrocyte F(ab′)2 considerably enhances their binding to erythrocytes in rat blood. However, no such increase in the binding was observed with rat leukocytes or monkey and mouse erythrocytes. Besides, the interactions between the liposomes and target cells did not affect the permeability properties of the liposome bilayer. These observations indicate that liposomes coupled to cell-specific antibodies may serve as highly useful carriers for homing of drugs/enzymes to specific cells in biophase. |
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