Occurrence and involvement of adenylate cyclase activity in the first step of tobacco mosaic virus infection of Nicotiana tabacum cv Xanthi nc leaves |
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| Affiliation: | 1. Station de Pathologie Végétale, I.N.R.A., B.P. 2078, 06606 Antibes, France;2. Laboratoire de Physiologie Cellulaire et Comparée and U.A. C.N.R.S. 651, Faculté des Sciences, 06034 Nice Cedex France;1. Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China;2. College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China;1. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;2. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China;1. State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China;2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;3. The Engineering Research Institute of Agriculture and Forestry, Ludong University, 186 Hongqizhong Road, Yantai, Shandong 264025, China |
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| Abstract: | Adenylate cyclase activity and its involvement in a physiopathological process (virus infection) were observed in a higher plant, Nicotiana tabacum cv Xanthi nc. The enzyme was characterized in leaves by measuring the conversion of [α-32P]ATP into cyclic [α-32P]AMP using a cell membrane preparation. The basal enzyme activity was 1–2 pmol/min per mg protein, was linear with time and protein concentration, and had a temperature optimum between 20 and 25% C. The Km for ATP was 2 mM in the presence or absence of stimulators. GTP (10−7 M) increased both basal and sodium fluoride-stimulated activities. During the hypersensitive reaction which follows tobacco mosaic virus (TMV) infection, we detected in the first 10 min a 40–80% increase in the basal activity. These results indicate that cAMP could play an important role by mediating the viral and plant host-cell interaction. The rapid pulse-release of cAMP leads us to propose that this nucleotide may, as in animal tissues, represent a secondary messenger in higher plants. |
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