Multiple hemoglobins in Triturus cristatus: their study by analytical isoelectrofocusing |
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Institution: | 1. University of Athens, Zoological Laboratory, Panepistimiopolis, Athens 15771, Greece;2. Nuclear Research Center “Demokritos”, Ag. Paraskevi, Attiki, Athens, Greece |
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Abstract: | - 1.1. Electrophoretic separation of the hemoglobin of healthy adult Triturus cristatus reveals four components.
- 2.2. Isoelectric focusing of the samee hemolysates in various commercial ampholytes of different chemical composition and pH range results in the separation of eight individual hemoglobin bands.
- 3.3. The bands obtained by electrophoresis are not homogeneous as revealed by individual gel electrofocusing. They finally separate into the same eight components, as in the whole hemolysate.
- 4.4. From the above findings it is concluded that this species has not four but eight individual hemoglobin molecular forms.
- 5.5. Our results demonstrate lack of hemoglobin polymorphism in this species.
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