Catechol dioxygenase expression in a Pseudomonas fluorescens strain exposed to different aromatic compounds |
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Authors: | G Cenci G Caldini |
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Institution: | (1) Dipartimento di Biologia Cellulare e Molecolare, Università di Perugia, Via del Giochetto, I-06126 Perugia, Italy. Fax: +39 75 5853328, IT |
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Abstract: | Batch cultures of Pseudomonas fluorescens (strain P2a) maintained under carbon-limiting conditions in the presence of chrysene and other aromatics, survived starvation
with no detectable changes in cell number for at least 4 months. P2a also demonstrated high dioxygenase levels after growth
on benzoate and catechol. To characterize this strain further, early stationary-phase cells were resuspended in fresh mineral
medium containing different aromatics, to evaluate enzyme expression in the presence of high-molecular-mass (isocyclic and
heterocyclic) compounds. Results demonstrated effects on catechol 1,2-dioxygenase modulation by the model compounds used,
confirming a low substrate specificity for this enzyme. The increases of specific activity observed in the presence of heterocyclic
compounds were higher than those observed with isocyclic compounds.
Received: 28 October 1996 / Accepted: 23 November 1996 |
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