| HCV NS5A基因表达及其在血清学检测中的应用评估 |
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| 引用本文： | 阮华,郜金荣,叶林柏,徐进平,王晓玲,赵月娥,吴正辉.HCV NS5A基因表达及其在血清学检测中的应用评估[J].中国病毒学,2001,16(2):190-192. |
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| 作者姓名： | 阮华 郜金荣 叶林柏 徐进平 王晓玲 赵月娥 吴正辉 |
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| 作者单位： | 武汉大学病毒研究所, |
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| 基金项目： | 湖北省重点基金资助项目(96290906) |
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| 摘 要： |
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| 关 键 词： | 丙型肝炎病毒 NS5A 表达 抗体检测 |
| 文章编号： | 1003-5125(2001)02-0190-03 |
| 修稿时间： | 2000年3月29日 |
Expression of Hepatitis C
Virus NS5A Gene In E.coli and Its Application in HCV Antibody Detection |
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| RUAN Hua,GAO Jin-rong,YE Lin-Bai,XU Jing-ping,WANG Xiao-ling,ZHAO Yue-e,WU Zheng-hui.Expression of Hepatitis C
Virus NS5A Gene In E.coli and Its Application in HCV Antibody Detection[J].Virologica Sinica,2001,16(2):190-192. |
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| Authors: | RUAN Hua GAO Jin-rong YE Lin-Bai XU Jing-ping WANG Xiao-ling ZHAO Yue-e WU Zheng-hui |
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| Abstract: | Full-length NS5A gene of the hepatitis C virus was amplified by PCR using plasmid pBAC25 containing HCV nonstructural gene as template. The amplified fragment (about 1.34 kb) was cloned into plasmid pQE32, and the recombinant plasmid pQENS5A was expressed in JM109 strain. The NS5A protein was purified by NiSO4 metal chelating resin, and characterized by Western-blot. Its antigenecity was determined by ELISA. The positive detection rate of anti-NS5A was 75% (69/92) in ninety-two clinic sera. The positive rate of anti-NS5A was 82.5% (33/40) in fourty positive standand sera, and the negative rate of anti-NS5A was 100% (40/40) in fourty negative standand sera. The results showed that the Full-length NS5A proteinn had the higher sensitivity and specificity in the detection of HCV antibody in sera, we suggested that NS5A protein was a useful antigen for blood screening. |
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