Purification and characterization of dermatan sulfate from the skin of the eel,Anguilla japonica |
| |
Authors: | Sakai Shinobu Kim Wan Seok Lee In Sun Kim Yeong Shik Nakamura Ayako Toida Toshihiko Imanari Toshio |
| |
Institution: | Department of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences, Chiba University, 263-8522, Chiba, Japan. |
| |
Abstract: | Glycosaminoglycans were isolated from the eel skin (Anguilla japonica) by actinase and endonuclease digestions, followed by a beta-elimination reaction and DEAE-Sephacel chromatography. Dermatan sulfate was the major glycosaminoglycan in the eel skin with 88% of the total uronic acid. The content of the IdoA2Salpha1-->4GalNAc4S sequence in eel skin, which shows anticoagulant activity through binding to heparin cofactor II, was two times higher than that of dermatan sulfate from porcine skin. The anti-IIa activity of eel skin dermatan sulfate was determined to be 2.4 units/mg, whereas dermatan sulfate from porcine skin shows 23.2 units/mg. The average molecular weight of dermatan sulfate was determined by gel chromatography on a TSKgel G3000SWXL column as 14 kDa. Based on 1H NMR spectroscopy, the presence of 3-sulfated and/or 2,3-sulfated IdoA residues was suggested. The reason why highly sulfated dermatan sulfate does not show anticoagulant activity is discussed. In addition to dermatan sulfate, the eel skin contained a small amount of keratan sulfate, which was identified by keratanase treatment. |
| |
Keywords: | Delmatan sulfate Eel skin Anguilla japonica 1H NMR Anti-factor IIa activity |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|