CAC2634-disrupted mutant of Clostridium acetobutylicum can be electrotransformed in air |
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Authors: | Dong H Tao W Zhu L Zhang Y Li Y |
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Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. |
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Abstract: | Aims: To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time‐consuming. Methods and Results: The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously constructed Restriction–Modification system deficient Cl. acetobutylicum mutant SMB009 was disrupted using ClosTron method. The resulted mutant SMB012 can be electrotransformed in air with an efficiency of 1·2–3·1 × 103 transformants μg?1 DNA. Conclusions: We demonstrated that the disruption of CAC2634 in Cl. acetobutylicum enables its electrotransformation in air. Significance and Impact of the Study: The electrotransformation process of Cl. acetobutylicum could be significantly simplified, especially when operating multiple electrotransformations. |
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Keywords: | CAC2634 Clostridium acetobutylicum electrotransformation in air simplified electrotransformation process |
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