| 应用逆转录PCR检测海产贝壳类中的甲型肝炎病毒 |
| |
| 引用本文： | 王稳,薄志坚,吕作芝,刘丹红,李小亮.应用逆转录PCR检测海产贝壳类中的甲型肝炎病毒[J].微生物学免疫学进展,2006,34(2):19-22. |
| |
| 作者姓名： | 王稳 薄志坚 吕作芝 刘丹红 李小亮 |
| |
| 作者单位： | 1. 大连市疾病预防控制中心,大连,116021
2. 兰州生物制品研究所,兰州,730046 |
| |
| 摘 要： | 污染水体中生长的贝壳类是传播甲型肝炎的重要媒介之一,本文介绍了用逆转录-聚合酶链反应(RT-PCR)检测贝壳类中甲型肝炎病毒(HAV)的方法。贝壳类中HAV的浓集回收采用洗脱-沉淀法并加以改进,30g贝肉中的病毒浓集回收在1.5~2.0m l上清液中。RT-PCR检出限为回收样品经二次扩增后10-2稀释度。从7种39份采自大连近海海域的贝壳类样品中,用该法检测出阳性样品4种10份,阳性率为26%。说明各种贝壳类有不同程度的污染。
|
| 关 键 词： | 逆转录-聚合酶链反应 甲型肝炎病毒 贝壳类 |
| 文章编号： | 1005-5673（2006）02-0019-04 |
| 收稿时间： | 2005-10-27 |
| 修稿时间： | 2006-01-24 |
Detection of HAV in Shellfish by RT-PCR |
| |
| WANG Wen,BO Zhi-jian,Lv Zuo-zhi,et al..Detection of HAV in Shellfish by RT-PCR[J].Progress In Microbiology and Immunology,2006,34(2):19-22. |
| |
| Authors: | WANG Wen BO Zhi-jian Lv Zuo-zhi |
| |
| Institution: | 1 Department of Microbiology , Dalian Center for Diseases Control and Prevention, Dalian 116021, China; 2 Lanzhou Institute of Biological Products, Lanzou 730046, China |
| |
| Abstract: | The consumption of Shellfish growing in contaminated waters is associated with the risk in transmission of HAV.We developed an RT-PCR method in detection of HAV in Shellfish(Arca suberenata,mussel and oyster et al).Initial virus extraction was based on a modified elution-precipitation technique.The detection limit of developed test was 10~(-2) dilution derived from Shellfish preparation by RT-PCR in second amplification,and 10 samples in four kinds out of 39 Shellfish samples in seven kinds from Dalian seashore were HAV positive.The general positive rate was 26%.The result showed that many kinds of Shellfish were contaminated by HAV. |
| |
| Keywords: | RT-PCR HAV Shellfish |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
