Synthesis of fluorescently labelled and internally quenched UDP-Gal probes |
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| Authors: | Schweizer Frank |
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| Institution: | Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2. schweize@cc.umanitoba.ca |
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| Abstract: | The preparation of fluorescently labelled and internally quenched UDP-Gal probes bearing a fluorescence emitter and a quencher is described. The rate of transfer using several galactosyltransferases was examined. Our results demonstrate that galactose-modified, sugar-nucleotide-modified and double modified UDP-Gal analogues are recognized as weak substrates by blood group B alpha-(1-->3) galactosyltransferase, alpha-(1-->3) galactosyltransferase and milk bovine beta-(1-->4) galactosyltransferase. |
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| Keywords: | Carbohydrates Nucleotides Glycosylations Galactosyltransferases Oligosaccharides UDP-Gal mimetics |
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