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Ten microsatellite loci for the strawberry poison frog (Oophaga pumilio) |
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| Authors: | J Susanne Hauswaldt Ann-Kathrin Ludewig Sabine Hagemann Heike Pröhl Miguel Vences |
| Institution: | 1. Unit of Evolutionary Biology, Institute of Zoology, Technical University of Braunschweig, Spielmannstr. 8, 38106, Braunschweig, Germany 2. Max-Planck Institute for Evolutionary Biology, Pl?n, Germany 3. Institute of Zoology, University of Veterinary Medicine, Hannover, Germany |
| Abstract: | We describe primers and PCR conditions to amplify nine new tetranucleotide loci and one new dinucleotide locus isolated from the strawberry poison frog (Oophaga pumilio). In 21 individuals from Costa Rica, the number of alleles ranged from 4 to 16, observed heterozygosities from 40 to 100%, and polymorphic information content ranged from 0.60 to 0.90 per locus. Evidence for linkage disequilibrium was found only between two loci, but this pattern was not found in other populations tested. All primer pairs cross-amplified in Oophaga vicentei from Panama. |
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