5-氨基水杨酸纳米粒对TNBS诱导小鼠结肠炎的治疗作用

目的:评价超临界分散法制备的5-氨基水杨酸(5-ASA)结肠定位纳米粒对小鼠结肠炎的治疗作用。方法:将24只6周龄BABL/c小鼠随机分为空白对照组,结肠炎模型组,5-ASA治疗组,5-ASA纳米粒治疗组。三硝基苯磺酸(TNBS)造模后的第2-7 d分别给予0.9%氯化钠,5-ASA和5-ASA纳米粒灌胃,于第8 d处死所有小鼠,评估小鼠的疾病活动指数(DAI),组织病理形态以及结肠组织学损伤情况;检测结肠组织髓过氧化物酶(MPO)活性。结果:5-ASA和5-ASA纳米粒均能明显减轻结肠重量,缩小溃疡面积,降低MPO活性。超临界分散法制备的5-ASA纳米粒在DAI,减轻局部的炎症程度(MPO水平)方面,其疗效与5-ASA组(50 mg/Kg)相当。结论:超临界分散法制备的5-ASA纳米粒对TNBS诱导小鼠的结肠炎有明显的治疗作用。

Effects of 5-ASA Nanoparticles on TNBS-Induced Colitis in Rats

Objective:To evaluate the effects of 5-ASA-nanoparticles (NP) on TNBS-induced colitis in rats.Methods:Twenty-four BALB/c rats were randomized into normal control group, colitis group, 5-ASA group, and 5-ASA-NP group, with six rats in each group. 5-ASA, 5-ASA-NP and normal saline solution were oral administrated respectively on day 2-7 after the establishment of colitis. On day 8, all animals were sacrificed. The change of body weight, weight to length ratio of colon, and macroscopic and pathologic score of colon were assessed. MPO activity of colonic tissue was measured.Results:Compared with normal group, the DAI, macroscopic and pathologic scores, MPO activity in colonic tissue in TNBS-control group increased significantly. In rats treated with 5-ASA or 5-ASA-NP, all the above-mentioned indices decreased significantly, and efficacy of 5-ASA-NP was almost the same as 5-ASA (50 mg/Kg).Conclusion:5-ASA-NP prepared by supercritical dispersion has significant effect on TNBS-induced colitis in rats with lower side effects, which suggested that it might be a potential therapeutic strategy for IBD.