八氢番茄红素合成酶(PSY)的生物信息学分析

目的:通过生物信息学方法对八氢番茄红素合成酶基因(PSY)及氨基酸序列分析,并构建三维结构。方法:运用生物信息学方法对八氢番茄红素合成酶基因及其蛋白质序列的理化性质、亲/疏水性、信号肽、跨膜结构域、糖基化位点,磷酸化位点,二级结构,功能结构域和三级结构进行预测分析。结果:PSY基因含1239bp的开放阅读框,编码氨基酸数为412,为碱性不稳定蛋白;八氢番茄红素合成酶富含Arg、Leu、Ala、Ser、Val等氨基酸,为亲水性蛋白质;PSY为非跨膜蛋白,不含信号肽,具有多个磷酸化位点,α螺旋和无规卷曲是其主要结构元件。结论:用同源建模的方法构建其三维结构,得到合理模型,为采用生物工程提高番茄红素产量提供理论依据。

Bioinformatic Analysis of Phytoene Synthase

Objective:To analyze the gene and amino acid sequence of eight hydrogen lycopene synthase (PSY) via bioinformatical methods, and build a three-dimensional structure.Methods:This research aimed to predict and analyze the physical and chemicalproperties, hydrophilicity and hydrophobicity, signal peptide, transmembrane domains, glycosylation and phosphorylation sites, secondary and tertiary structure of PSY gene and protein sequence by bioinformatic methods.Results:PSY gene is an alkali labile protein,which contains a long open reading frame of 1 239bp, encodes 412 amino acid; PSY is hydrophilic protein, which is rich in Arg, Leu, Ala,Ser, Val etc; PSY is non-transmembrane protein, which has no signal peptide and contains several phosphorylation sites, alpha helix and random coil is its primary secondary structural elements.Conclusion:The reasonable three-dimensional structure of PSY was obtained byusing homology modeling software.The research offer a theoretical basis to improve the yield of lycopene in biotechnology.