Ethanol extraction requirement for purification of protein labeled with [h]leucine in aquatic bacterial production studies |
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| Authors: | Wicks R J Robarts R D |
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| Institution: | Division of Water Technology, Council for Scientific and Industrial Research, P. O. Box 395, Pretoria 0001, South Africa. |
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| Abstract: | The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is H]leucine. Labeled-protein purification requires an ethanol wash step. Cold TCA can replace hot TCA for precipitation of labeled proteins. |
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