Toxicity of Bacillus thuringiensis Spo Cr Mutants for the European Corn Borer Ostrinia nubilalis |
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Authors: | Johnson D E Freedman B |
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Affiliation: | Northern Regional Research Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Peoria, Illinois 61604. |
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Abstract: | Inclusion bodies isolated from Spo Cr mutants of Bacillus thuringiensis were toxic for larvae of the European corn borer. Probit analysis revealed comparable toxicity between wild-type crystals (isolated from B. thuringiensis subsp. kurstaki) and crystals produced from two spore-free mutants of the same subspecies. Death of the larvae was due to starvation, presumably through delta-endotoxin-induced gut paralysis. Inclusion bodies pretreated with alpha-chymotrypsinogen were equally as toxic as native crystals for the insect larvae. |
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