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Medium for Screening Leuconostoc oenos Strains Defective in Malolactic Fermentation
Authors:Cavin J F  Prevost H  Lin J  Schmitt P  Divies C
Institution:ENS.BANA, Laboratoire de Microbiologie, Université de Bourgogne, Campus Universitaire Montmuzard, 21000 Dijon, France.
Abstract:A new sensitive medium was developed to screen and isolate mutagenic Leuconostoc oenos strains defective in malolactic fermentation. The essential components of the medium included fructose (22 mM), l-malic acid (74.6 mM), bromocresol green (as pH indicator), and cellulose powder. The wild-type colonies turned blue, but defective malolactic colonies gave an acid reaction and remained yellow-green.
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