Detection of beta-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate |
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| Authors: | Kwon K S Lee J Kang H G Hah Y C |
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| Affiliation: | Department of Microbiology, Seoul National University, Seoul, 151-742, Korea. |
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| Abstract: | beta-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by beta-glucosidase action reacts with ferric ion to produce a black band, corresponding to the beta-glucosidase, against the transparent background. |
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