Occurrence of an Affinity Site apart from the Active Site on the Raw-Starch-Digesting but Non-Raw-Starch-Adsorbable Bacillus subtilis 65 alpha-Amylase |
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| Authors: | Hayashida Shinsaku Teramoto Yuji Inoue Takehiro Mitsuiki Shinji |
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| Institution: | Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan. |
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| Abstract: | alpha-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 alpha-amylase. The raw starch digestibility and alpha-cyclodextrin-Sepharose 6B adsorbability of this alpha-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-digesting enzymes was proven also with bacterial amylase. |
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