Fabrication of nanofiber coated with l-arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells |
| |
| Authors: | Sivakumar Allur Subramaniyan Sunirmal Sheet Saravanakumar Balasubramaniam Swami Vetha Berwin Singh Dileep Reddy Rampa Sureshkumar Shanmugam |
| |
| Affiliation: | 1. Department of Animal Biotechnology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju-si, Republic of Korea;2. Department of Wood Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju-si, Republic of Korea;3. School of Chemistry and Environment, South China Normal University, Guangshou, China;4. Molecular Imaging and Therapeutic Medicine Research Center, Cyclotron Research Center, Research Institute of Clinical Medicine, Biomedical Research Institute, Chonbuk National University, Medical School and Hospital, Jeonju-si, Republic of Korea;5. Department of BIN convergence Technology, College of Engineering, Chonbuk National University, Jeonju, Republic of Korea;6. Department of Animal Science and BK21 PLUS program, Jeonju-si, Republic of Korea |
| |
| Abstract: | The objective of this study was to synthesize and characterize novel polyurethane (PU)-nanofiber coated with l-arginine by electrospinning technique. This study determined whether l-arginine conjugated with PU-nanofiber could stimulate cell proliferation and prevent H2O2-induced cell death in satellite cells co-cultured with fibroblasts isolated from Hanwoo (Korean native cattle). Our results showed that l-arginine conjugated with PU nanofiber could reduce cytotoxicity of co-cultured satellite cells. Protein expression levels of bcl-2 were significantly upregulated whereas those of caspase-3 and caspase-7 were significantly downregulated in co-culture of satellite cells compared to those of monoculture cells after treatment with PU-nanofiber coated with l-arginine and which confirmed by Confocal microscope. These results suggest that co-culture of satellite cells with fibroblasts might be able to counter oxidative stress through translocation/penetration of antioxidant, collagen, and molecules secreted to satellite cells. Therefore, this nanofiber might be useful as a wound dressing in animals to counter oxidative stresses. |
| |
| Keywords: | Co-culture cell–cell communication nanofiber oxidative stress and apoptosis |
|
|
