Control of a Nanoscopic-to-Macroscopic Transition: Modulated Phases in Four-Component DSPC/DOPC/POPC/Chol Giant Unilamellar Vesicles |
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| Authors: | Tatyana M. Konyakhina,Shih Lin Goh,Jonathan Amazon,Frederick A. Heberle,Jing Wu,Gerald W. Feigenson |
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| Affiliation: | †Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York;‡Neutron Scattering Science Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee;§Department of Chemistry, University of Michigan, Ann Arbor, Michigan |
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| Abstract: | We have found modulated phase morphology in a particular region of composition within the liquid-ordered + liquid-disordered coexistence region in the four-component lipid bilayer mixture DSPC/DOPC/POPC/Chol. By controlling lipid composition, we could see distinct types of modulated liquid-liquid phase morphologies, including linear, irregular, and angular features in giant unilamellar vesicles. We used a combination of confocal, two-photon, wide-field fluorescence, and differential interference contrast microscopies, and used stringent controls to minimize light-induced artifacts. These studies establish that both the size and morphology of membrane rafts can be controlled by the concentration and the type of low-melting lipid in mixtures with cholesterol and a high-melting lipid. |
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