The accuracy of poly(U) translation by different eukaryotic tRNAs |
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Authors: | A V El'skaya A P Soldatkin |
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Affiliation: | Institute of Molecular Biology and Genetics, The Ukrainian SSR Academy of Sciences, Kiev 252143, USSR |
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Abstract: | In this work we present comparative data on rates of phenylalanine and leucine incorporation into the poly(U) dependent product of cell-free translation by different eukaryotic tRNAs at high Mg2+ concentration. The frequency of translation errors has been found to depend upon the value of the tRNAPhe:tRNALeu ratio and the peculiarities of isoacceptor tRNAsLeu of different origin. |
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Keywords: | Translation accuracy Poly(U) misreading Aminoacyl-tRNA competition |
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