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The accuracy of poly(U) translation by different eukaryotic tRNAs
Authors:A V El'skaya  A P Soldatkin
Affiliation:Institute of Molecular Biology and Genetics, The Ukrainian SSR Academy of Sciences, Kiev 252143, USSR
Abstract:In this work we present comparative data on rates of phenylalanine and leucine incorporation into the poly(U) dependent product of cell-free translation by different eukaryotic tRNAs at high Mg2+ concentration. The frequency of translation errors has been found to depend upon the value of the tRNAPhe:tRNALeu ratio and the peculiarities of isoacceptor tRNAsLeu of different origin.
Keywords:Translation accuracy  Poly(U) misreading  Aminoacyl-tRNA competition
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