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| 东北虎幼体消化系统蛋白水解酶的初步研究 | |
| 引用本文： | 牛红星,卜艳珍,余燕,姬生栋,王艳梅,卢全伟,刘敬泽.东北虎幼体消化系统蛋白水解酶的初步研究[J].兽类学报,2006,26(1):98-100. |
| 作者姓名： | 牛红星 卜艳珍 余燕 姬生栋 王艳梅 卢全伟 刘敬泽 |
| 作者单位： | 1. 河北师范大学生命科学学院,石家庄,050016;河南师范大学生命科学学院,新乡,453007 2. 河南师范大学生命科学学院,新乡,453007;河南科技学院动物科学系,新乡,453003 3. 河南师范大学生命科学学院,新乡,453007 4. 河北师范大学生命科学学院,石家庄,050016 |
| 基金项目： | 河南省自然科学基金资助项目(0411032500)，河南省动物学省级重点学科经费资助 |
| 摘 要： | 蛋白水解酶在许多生命活动中是必需的物质(Vassalli and Pepper,1994)。蛋白质的酶解修饰(Xuet al.,1999)、细胞迁移、组织再生与修复、消化系统对蛋白质的消化等均与蛋白水解酶有关(Baimbridgeet al.,1992),且蛋白水解酶功能失调会导致许多疾病(Teichertet al.,1989)。东北虎( |
| 关 键 词： | 东北虎幼体 消化系统 蛋白水解酶 复性电泳 |
| 文章编号： | 1000-1050（2006）01-0098-03 |
Preliminary studies on proteolytic enzymes in the digestive system of Amur tiger cubs(Panthera tigris altaica) |
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| NIU Hongxing,BU Yanzhen,YU Yan,JI Shengdong,WANG Yanmei,LU Quanwei,LIU Jingze.Preliminary studies on proteolytic enzymes in the digestive system of Amur tiger cubs(Panthera tigris altaica)[J].Acta Theriologica Sinica,2006,26(1):98-100. | |
| Authors: | NIU Hongxing BU Yanzhen YU Yan JI Shengdong WANG Yanmei LU Quanwei LIU Jingze |
| Institution: | 1. College of Life Sciences, Hebei Normal University, Shijiazhuang , 050016, China;2. College of Life Sciences, Henan Normal University, Xinxiang , 453007, China ; 3. Department of Animal Sciences, Henan Institute of Science Technology, Xinxiang, 453003, China |
| Abstract: | The kinds and activities of proteolytic enzymes in the digestive systems (tongue, esophagus, stomach, duodenum, liver, pancreas and large intestine) of two Amur tiger cubs (Panthera tigris altaica) were investigated using protein detection and SDS-G-PAGE. The results showed:1) the digestive system of Amur tiger cubs had 30 different kinds of proteolytic enzymes ; 2) the mostly optimal pH for these enzymes was a neutral environment, and an acid condition highly restrained their activity; 3) the proteolytic enzymes in the tongue and esophagus were fewer than in other digestive organs and their activities were weak as well; 4) the duodenum had the most kinds of proteolytic enzymes with the strongest activities under various pH conditions; 5) the 16 kD enzyme bands always existed under neutral and alkalescence pH conditions in all digestive organs except the tongue. |
| Keywords: | Digestive system Panthera tigris altaica Proteolytic enzymes SDS-G-PAGE |
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