马尾松枝条挥发性组分的鉴定及松墨天牛对其触角电生理反应

为研究松墨天牛Monochamus alternatus Hope取食期引诱剂,利用气相色谱-质谱(GC-MS)联用技术并与标样核对分析了马尾松(Pinus massoniana Lamb.)枝条挥发性气味的化学组成。结果表明,马尾松1年生、2年生、3年生枝条气味均由8种萜烯类物质组成,但各组分相对含量不同。经方差分析,α-蒎烯、莰烯、β-蒎烯、柠檬烯、萜品油烯、β-石竹烯在3种枝条中的相对百分含量的差异均达到极显著水平。1年生枝条中α-蒎烯、莰烯和萜品油烯的相对含量最小,分别为20.81%、0.43%和4.74%,而β-蒎烯、月桂烯和柠檬烯的相对含量最高,分别为12.37%、4.33%和4.53%。鉴定的化合物对松墨天牛雌雄成虫的触角电生理反应的实验,结果表明,松墨天牛雌雄成虫对α-蒎烯的反应最强,对β-石竹烯的反应最弱。

Volatiles from wattles of Pinus massoniana and EAG responses of the adult Monochamus alternatus to the identified chemicals

The volatiles from wattles of Pinus massoniana Lamb. were determined by gas chromatography-mass spectrometry (GC-MS). The results showed that there were eight constituents in volatiles of one-, two-and three-year-old wattles of P. massoniana, which were consisted of α-pinene, camphene, β-pinene, myrcene, limonene, β-phellandrene, terpinolene and β-caryophyllene. But relative contents of these chemicals varied with wattle ages, with considerable difference in α-pinene, camphene, β-pinene, limonene, terpinolene and β-caryophyllene. The relative contents of α-pinene, camphene and terpinolene in one-year-old wattles were the least, which was 20.81%, 0.43% and 4.74% respectively. Whereas, the relative contents of β-pinene, myrcene and limonene were the most, which was 12.37%, 4.33% and 4.53% respectively. Electroantennogram (EAG) responses of the adult Monochamus alternatus Hope to these identified chemicals were tested and α-pinene caused maximum EAG responses, and β-caryophyllene the least.