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Phenotypic and genotypic identification of bacteria from the Burkholderia cepacia complex
Authors:Antonov V A  Iliukhin V I  Senina T V  Loboĭko A D  Tkachenko G A  Zinchenko O V  Mazurova I Iu
Abstract:AIM: Evaluation of the diagnostic value of pheno- and genotypic characteristics of B. cepacia strains collection. MATERIALS AND METHODS: Phenotypic and genetic methods of identification and differentiation of 25 strains of the B. cepacia complex. RESULTS: Polyphasic taxonomic approach utilizing multiple diagnostic tests was used for accurate identification of Burkholderia species. Algorithm for identification of microorganisms from the B. cepacia complex was developed. CONCLUSION: Combined use of phenotypic and molecular genetic tests, such as recA gene PCR, is recommended for differentiation of the B. cepacia complex genomovars.
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