Sialo proteins and phosphatases in estrual cervical mucus of fertile and repeat breeder buffaloes (Bubalus bubalis )

Concentrations of sialic acid and protein and activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) were determined in estrual cervical mucus of fertile and repeat breeder buffaloes during late estrus. Significantly (P < 0.01) higher concentrations of sialic acid and ACP and AKP activities were observed in fertile buffaloes as compared with repeat breeder buffaloes. However, differences were nonsignificant in protein content between fertile and repeat breeder buffaloes, albeit protein concentration was higher in fertile buffaloes. The significance of sialoproteins and phosphatases in fertility has been clarified.