Regulation of avilamycin biosynthesis in Streptomyces viridochromogenes: effects of glucose, ammonium ion, and inorganic phosphate |
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Authors: | Chuan-he Zhu Fu-ping Lu Ya-nan He Zhen-lin Han Lian-xiang Du |
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Institution: | (1) Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, 30022 Tianjin, People’s Republic of China;(2) College of Food Science and Engineering, Shangdong Agriculture University, 271018 Taian, People’s Republic of China |
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Abstract: | Effects of glucose, ammonium ions and phosphate on avilamycin biosynthesis in Streptomyces viridochromogenes AS4.126 were investigated. Twenty grams per liter of glucose, 10 mmol/L ammonium ions, and 10 mmol/L phosphate in the basal medium
stimulated avilamycin biosynthesis. When the concentrations of glucose, ammonium ions, and phosphate in the basal medium exceeded
20 g/L, 10 mmol/L, and 10 mmol/L, respectively, avilamycin biosynthesis greatly decreased. When 20 g/L glucose was added at
32 h, avilamycin yield decreased by 70.2%. Avilamycin biosynthesis hardly continued when 2-deoxy-glucose was added into the
basal medium at 32 h. There was little influence on avilamycin biosynthesis with the addition of the 3-methyl-glucose (20 g/L)
at 32 h. In the presence of excess (NH4)2SO4 (20 mmol/L), the activities of valine dehydrogenase and glucose-6-phosphate dehydrogenase were depressed 47.7 and 58.3%,
respectively, of that of the control at 48 h. The activity of succinate dehydrogenase increased 49.5% compared to the control
at 48 h. The intracellular adenosine triphosphate level and 6-phosphate glucose content of S. viridochromogenes were 128 and 129%, respectively, of that of the control at 48 h, with the addition of the 40 mmol/L of KH2PO4. As a result, high concentrations of glucose, ammonium ions, and inorganic phosphate all led to the absence of the precursors
for avilamycin biosynthesis and affected antibiotic synthesis. |
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Keywords: | Regulation Biosynthesis Avilamycin Streptomyces viridochromogenes AS4 126 |
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