Flow cytometric DNA analysis of nurse shark, Ginglymostoma cirratum (Bonaterre) and clearnose skate, Raja eglanteria (Bosc) peripheral red blood cells |
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| Authors: | C. Kendall,S. Valentino,&dagger ,A. B. Bodine,&dagger C. A. Luer,&Dagger |
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| Affiliation: | Poultry Science Department, Clemson University, Clemson, SC 29634-0379, U.S.A.;Animal, Dairy and Veterinary Sciences Department, Clemson University, Clemson, SC 29634-0379 U.S.A.;Mote Marine Laboratory, Sarasota, FL 33577, U.S.A. |
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| Abstract: | Flow cytometric DNA analysis was used to determine the DNA content of red blood cells from Ginglymostoma cirratum (Bonaterre) and Raja eglanteria (Bosc). The DNA content/nucleus was calculated to be 7.6 and 7.1 pg/nucleus, respectively. Peripheral red blood cells obtained both from G. cirratum and from R. eglanteria were demonstrated to be actively cell cycling. Percentages of red blood cells in the phases of the cell cycle ( G 0/ G 1, S , and G 2/ M ) not only varied considerably between different fishes but also were quite variable in weekly samples obtained from individual animals. |
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| Keywords: | elasmobranch DNA analysis flow cytometer red blood cells Ginglymostoma cirratum Raja eglanteria |
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