Characterization of peptidoglycan stem lengths by solid-state 13C and 15N NMR |
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| Authors: | J Schaefer J R Garbow G S Jacob T M Forrest G E Wilson |
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| Affiliation: | 1. Monsanto Company, Physical Sciences Center, St. Louis, MO 63167 USA;2. Department of Chemistry, University of Akron, Akron, OH 44325 USA;1. Mayo Clinic School of Medicine, Rochester, Minn;2. Department of Radiology, Mayo Clinic, Rochester, Minn;3. Department of Science of Healthcare Delivery, Mayo Clinic, Rochester, Minn;4. Department of Neurosurgery, Mayo Clinic, Rochester, Minn;1. Center for Translational Medicine, First Affiliated Hospital of Xi''an Jiaotong University, School of Medicine, Xi''an, Shaanxi, China;2. Department of Pharmacy, Henan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China;3. Department of Sports Medicine, Henan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital), Luoyang, Henan, China;1. Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, Medical Research Center, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, Hunan, 421001, China;2. Department of Cardiovascular Medicine, The Second Affiliated Hospital of University of South China, Hengyang, 421001, Hunan, China;3. Department of Biochemistry and Molecular Biology, The Libin Cardiovascular Institute of Alberta, The University of Calgary, Health Sciences Center, 3330 Hospital Dr NW, Calgary, Alberta, T2N 4N1, Canada;1. Department of Applied Bioorganic Chemistry, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan;2. Center for Highly Advanced Integration of Nano and Life Sciences (G-CHAIN), Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan |
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| Abstract: | Lyophilized whole cells of Aerococcus viridans (Gaffkya homari) grown on a synthetic medium containing D-[2-13C, 15N]Ala, or containing both L-[1-13C]Lys and D-[15N]Ala, have been examined by double cross-polarization magic-angle spinning 13C and 15N nuclear magnetic resonance. Results from the double-labeled alanine experiment confirm the absence of metabolic scrambling of alanine by A. viridans. Results from the combined single-label experiment can be used to count directly the number of adjacent L-Lys and D-Ala units in peptide chains of cell-wall peptidoglycan. This count leads to the conclusion that there are no terminal D-Ala or D-Ala-D-Ala units in uncross-linked chains of the peptidoglycan of A. viridans. |
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