Sequence analysis of a cDNA containing the gag and prot regions of the soybean retrovirus-like element, SIRE-1 |
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Authors: | Yi-An Bi Howard M. Laten |
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Affiliation: | (1) Department of Biology, Loyola University Chicago, 6525 N. Sheridan Road, 60626 Chicago, IL, USA |
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Abstract: | SIRE-1 is a family of several hundred dispersed copies of a very large DNA element from Glycine max that has features characteristic of retroviruses and retrotransposons. A 2.4 kb SIRE-1-specific fragment was recovered from a soybean cDNA library and sequenced. The sequence contains two ORFs. Theoretical translation of ORF1 produces a gag-prot-like polyprotein containing highly conserved motifs found in retroelement nucleocapsids (CX2CX4HX4C) and aspartic proteases (LDSG). The second ORF is foreshortened. The cDNA also contains nearly 200 bp of a putative 5 LTR just upstream of a tRNA primer-binding site. |
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Keywords: | aspartic protease copia-like gag LTR retrotransposon retrovirus-like soybean |
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