The effect of n-propyl gallate on the formation of ethylene during protoplast isolation in sugarbeet (Beta vulgaris L.) |
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Authors: | Krens, F.A. Jamar, D. Keizer, L.C.P. Hall, R.D. |
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Abstract: | The formation of ethylene during isolation of mesophyll protoplastsfrom leaves of in vitro cultured sugarbeet (Beta vulgaris L.)seedlings was monitored. The addition of the lipoxygenase (EC1.13.11.12[EC]) inhibitor n-propyl gallate to the isolation mediumsignificantly reduced the amount and rate of ethylene production.The relevance of cell physiology on protoplast regenerationis discussed. Key words: Beta vulgaris L., protoplasts, ethylene, lipoxygenase inhibitor, regeneration |
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