A disbalance between beta-adrenergic and muscarinic responses caused by hydrogen peroxide in rat airways in vitro |
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| Authors: | K Kramer C J Doelman H Timmerman A Bast |
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| Affiliation: | 1. The Menninger Clinic, 12301 S. Main Street, Houston, TX 77035, USA;2. The University of Houston, 4800 Calhoun Road, Houston, TX 77004, USA;1. Department of Internal Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile;2. Hospital Alemán, Buenos Aires, Argentina;3. Servicio de Hematología, Complejo Hospitalario Dr. Arnulfo Arias Madrid, Panama City, Panama;4. Clínica Universitaria Colombia, Bogotá, Colombia;5. Epidemiología Clínica y Evidencia, Instituto de Investigaciones en Salud Pública, Universidad de Buenos Aires, Buenos Aires, Argentina;6. Centro de Hematología Pavlovsky, Buenos Aires, Argentina;7. Fundación Favaloro, Buenos Aires, Argentina;8. Hospital de Clínicas Dr. Manuel Quintela, Montevideo, Uruguay;9. Faculty of Medicine, Universidad Privada Antenor Orrego, Trujillo, Peru;10. Hospital Especializado Víctor Lazarte Echegaray, Trujillo, Peru;11. The ABC Medical Center, Mexico City, Mexico;12. Department of Hematology and Oncology, Pontificia Universidad Católica de Chile, Santiago, Chile;13. Department of Internal Medicine, Faculty of Medicine Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;14. Unidad Hematológica Especializada IPS, Cucuta, Colombia;15. Caja Petrolera de Salud, La Paz, Bolivia;16. School of Health and Related Research, University of Sheffield, Sheffield, United Kingdom;17. Department of Pediatrics, Pontificia Universidad Católica de Chile, Santiago, Chile;18. Department of Health Research Methods, Evidence and Impact, McMaster University, Hamilton, ON, Canada;1. Department of Physics and Astronomy, University of Calgary, 2500 University Drive North West, Calgary, AB, Canada T2N 1N4;2. Department of Physics and Astronomy, University of British Columbia, 6224 Agricultural Road, Vancouver, BC, Canada V6T 1Z1;3. Space Sciences Laboratory and Department of Physics, University of California, Berkeley, CA 94720, USA;4. Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA;5. Department of Physics, The College of William and Mary, Williamsburg, VA 23187, USA |
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| Abstract: | The effect of hydrogen peroxide on adrenergic and muscarinic responses of rat airway smooth muscle was studied. The trachea muscle and the lung parenchymal strip were contracted with methacholine and relaxed with (-)-isoprenaline. Recording of three (-)-isoprenaline curves on the trachea muscle and the lung parenchymal strip was followed by treatment for 30 min with hydrogen peroxide (H2O2) (1mM) after which a new dose response curve for (-)-isoprenaline was constructed. Using the trachea muscle this treatment with H2O2 resulted in a decrease of 61% of the maximum contraction by methacholine compared with the control and a complete inhibition of the relaxation by (-)-isoprenaline. In the lung parenchymal strip preparation we found, after the same treatment no reduction of the contraction by methacholine and 61% reduction of the relaxation by (-)-isoprenaline, compared with the control. The results demonstrate that the adrenergic response in rat airways is more susceptible to hydrogen peroxide than the muscarinic response. |
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