一种新的与细胞分化相关的基因在不同组织及细胞系中表达与定位

目的　研究一种新的可能与细胞分化相关的基因在正常组织、肿瘤组织及细胞系中的表达与定位 ,初步探讨该基因的作用机制。方法　利用Northern杂交方法检测 10种人胎儿组织、6种人肿瘤细胞系、4种人的肿瘤组织及癌旁组织中该基因的表达。利用免疫荧光实验检测该基因在细胞中定位。结果　该基因在人的胎儿组织及肿瘤组织和肿瘤细胞中均有高表达 ,在正常组织及癌旁组织中表达明显减弱。癌旁组织和癌组织中的表达差异有显著性 (P <0 0 5 )。该基因在K56 2 细胞中主要定位在膜上。结论　该基因可能在细胞分化及肿瘤发生中起着重要作用

Expression and Location of a Novel Gene with Cell Differentiation in Different Tissues and Cell Lines

Objective To study expression and location of a novel gene that might be related to cell differentiation in normal tissues,cancer tissues and cell lines.Methods\ Northern blotting method was used to detect the expression of the gene in 10 embryo tissues,6 cancer cell lines,4 cancer tissues.The immunohistochimical method was used to detect the gene location.Results\ The gene showed high level expression in embryo tissue,cancer tissue and cell line of human,the expression in normal tissue and paratumor tissue decrease obviously.The gene is located on the membrane of K 562 cell.Conclusion\ The gene may play an important role in cell differentiation and carcinogenesis.