A simple method for the quantitative evaluation of functional effects of xenobiotics with cultured cells |
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Authors: | D. Lees D. Pugnère A. Castel O. Raux P. Travo |
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Affiliation: | (1) Centre de Recherches de Biochimie Macromoléculaire, UPR 9008 CNRS, U 249 INSERM, Université de Montpellier I, BP 5051, 34033 Montpellier Cédex, France |
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Abstract: | We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time intervals ranging from 10 s to 6 h or more, over periods of time ranging from a few tens of minutes to 3–4 days. Any aspect of cell physiological behavior can be studied (differentiation-dedifferentiation, migration, division, degeneration, death) without preliminary staining and/or fixation provided it results in optically visible changes. |
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Keywords: | cell cultures single-cell functional quantitative studies noninvasive nondestructive image analysis |
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