Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation |
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| Authors: | Adamski Danièle Mayol Jean-François Platet Nadine Berger François Hérodin Francis Wion Didier |
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| Affiliation: | a INSERM U836, CHU Michallon, 38043 Grenoble, France
b Centre de Recherches du Service de Santé des Armées, P.O. Box 87, 38702 La Tronche, France |
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| Abstract: | Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation of data. |
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| Keywords: | Side population Hoechst 33342 Cancer stem cell Stem cell |
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