Identification and characterization of DUSP27, a novel dual-specific protein phosphatase |
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Authors: | Friedberg Ilan Nika Konstantina Tautz Lutz Saito Kan Cerignoli Fabio Friedberg Iddo Godzik Adam Mustelin Tomas |
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Affiliation: | The Burnham Institute for Medical Research, North Torrey Pines Road, La Jolla, CA 92037, USA |
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Abstract: | A novel human dual-specific protein phosphatase (DSP), designated DUSP27, is here described. The DUSP27 gene contains three exons, rather than the predicted 4-14 exons, and encodes a 220 amino acid protein. DUSP27 is structurally similar to other small DSPs, like VHR and DUSP13. The location of DUSP27 on chromosome 10q22, 50 kb upstream of DUSP13, suggests that these two genes arose by gene duplication. DUSP27 is an active enzyme, and its kinetic parameters and were determined. DUSP27 is a cytosolic enzyme, expressed in skeletal muscle, liver and adipose tissue, suggesting its possible role in energy metabolism. |
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Keywords: | DiFMUP, 6,8-difluoro-4-methylumbelliferyl phosphate DSP, dual-specific protein phosphatase DUPD1, dual-specific protein phosphatase domain containing 1 DUSP27, dual-specific phosphatase 27 His6-DUSP, histidine epitope-tagged DUSP27 HA-DUSP27, hemagglutinin epitope-tagged DUSP27 InsP, inositol-phosphate pNPP, p-nitrophenyl phosphate PTK, protein tyrosine kinase PTP, protein tyrosine phosphatase VHR, VH1 related |
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