A rapid technique for screening of lovastatin-producing strains of Aspergillus terreus by agar plug and Neurospora crassa bioassay |
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Authors: | Kumar M S Kumar P M Sarnaik H M Sadhukhan A K |
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Affiliation: | Fermentation Laboratory, Biotechnology R&D, Dr. Reddy's Research Foundation, Miyapur, Hyderabad, India. |
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Abstract: | The success of strain improvement programme depends on the number of isolates that can be screened after mutagenic treatment. A technique to rapidly screen large number of high-yielding isolates was developed. The 'agar plug' method that utilizes the anti-fungal property of lovastatin to produce a zone of inhibition against Neurospora crassa was not only economical but also less labour-intensive. We were able to isolate a high-yielding strain, the productivity of which increased by 138% as compared to the parent strain in the submerged fermentation process. |
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