Cyclin‐dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity |
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Authors: | Christian P Craddock Nicolette Adams Johan TM Kroon Fiona M Bryant Patrick J Hussey Smita Kurup Peter J Eastmond |
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Institution: | 1. School of Life Sciences, University of Warwick, Coventry, UK;2. School of Biological and Biomedical Sciences, Durham University, Durham, UK;3. Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire, UK |
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Abstract: | Coordination of endomembrane biogenesis with cell cycle progression is considered to be important in maintaining cell function during growth and development. We previously showed that the disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in Arabidopsis thaliana stimulates biosynthesis of the major phospholipid phosphatidylcholine (PC) and causes expansion of the endoplasmic reticulum. Here we show that PC biosynthesis is repressed by disruption of the core cell cycle regulator CYCLIN‐DEPENDENT KINASE A;1 (CDKA;1) and that this repression is reliant on PAH. Furthermore, we show that cyclin‐dependent kinases (CDKs) phosphorylate PAH1 at serine 162, which reduces both its activity and membrane association. Expression of a CDK‐insensitive version of PAH1 with a serine 162 to alanine substitution represses PC biosynthesis and also reduces the rate of cell division in early leaf development. Together our findings reveal a physiologically important mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis to cell cycle progression in Arabidopsis. |
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Keywords: |
Arabidopsis thaliana
phosphatidylcholine phospholipid biosynthesis membrane biogenesis phosphatidic acid phosphohydrolase phosphorylation cell cycle cyclin‐dependent kinase |
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