The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP‐dependent downstream signaling |
| |
| Authors: | Janet I. Wheeler Aloysius Wong Claudius Marondedze Arnoud J. Groen Lusisizwe Kwezi Lubna Freihat Jignesh Vyas Misjudeen A. Raji Helen R. Irving Chris Gehring |
| |
| Affiliation: | 1. Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, VIC, Australia;2. AgriBio, La Trobe University, Bundoora, VIC, Australia;3. Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia;4. College of Natural, Applied and Health Sciences, Wenzhou‐Kean University, Ouhai, Wenzhou, Zhejiang Province, China;5. Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, UK;6. Council for Scientific and Industrial Research, Biosciences, Pretoria, South Africa;7. Analytical Chemistry Core Laboratory, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia |
| |
| Abstract: | The brassinosteroid receptor brassinosteroid insensitive 1 (BRI1) is a member of the leucine‐rich repeat receptor‐like kinase family. The intracellular kinase domain of BRI1 is an active kinase and also encapsulates a guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that the recombinant cytoplasmic domain of BRI1 generates pmol amounts of cGMP per μg protein with a preference for magnesium over manganese as a co‐factor. Importantly, a functional BRI1 kinase is essential for optimal cGMP generation. Therefore, the guanylate cyclase activity of BRI1 is modulated by the kinase while cGMP, the product of the guanylate cyclase, in turn inhibits BRI1 kinase activity. Furthermore, we show using Arabidopsis root cell cultures that cGMP rapidly potentiates phosphorylation of the downstream substrate brassinosteroid signaling kinase 1 (BSK1). Taken together, our results suggest that cGMP acts as a modulator that enhances downstream signaling while dampening signal generation from the receptor. |
| |
| Keywords: |
Arabidopsis thaliana
brassinosteroid receptor (BRI1)
cGMP
kinase guanylate cyclase brassinosteroid signaling kinase 1 (BSK1) |
|
|
