A photo‐responsive F‐box protein FOF2 regulates floral initiation by promoting FLC expression in Arabidopsis |
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Authors: | Reqing He Xinmei Li Ming Zhong Jindong Yan Ronghuan Ji Xu Li Qin Wang Dan Wu Mengsi Sun Dongying Tang Jianzhong Lin Hongyu Li Bin Liu Hongtao Liu Xuanming Liu Xiaoying Zhao Chentao Lin |
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Affiliation: | 1. Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, China;2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China;3. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;4. Department of Molecular, Cell & Developmental Biology, University of California, Los Angeles, CA, USA |
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Abstract: | Floral initiation is regulated by various genetic pathways in response to light, temperature, hormones and developmental status; however, the molecular mechanisms underlying the interactions between different genetic pathways are not fully understood. Here, we show that the photoresponsive gene FOF2 (F‐box of flowering 2) negatively regulates flowering. FOF2 encodes a putative F‐box protein that interacts specifically with ASK14, and its overexpression results in later flowering under both long‐day and short‐day photoperiods. Conversely, transgenic plants expressing the F‐box domain deletion mutant of FOF2 (FOF2ΔF), or double loss of function mutant of FOF2 and FOL1 (FOF2‐LIKE 1) present early flowering phenotypes. The late flowering phenotype of the FOF2 overexpression lines is suppressed by the flc‐3 loss‐of‐function mutation. Furthermore, FOF2 mRNA expression is regulated by autonomous pathway gene FCA, and the repressive effect of FOF2 in flowering can be overcome by vernalization. Interestingly, FOF2 expression is regulated by light. The protein level of FOF2 accumulates in response to light, whereas it is degraded under dark conditions via the 26S proteasome pathway. Our findings suggest a possible mechanistic link between light conditions and the autonomous floral promotion pathway in Arabidopsis. |
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Keywords: | F‐box protein FOF2
FLOWERING LOCUS C
flowering light autonomous pathway
Arabidopsis thaliana
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